3S16
RNA Polymerase II Initiation Complex with an 8-nt RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-30 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 157.843, 221.125, 192.746 |
Unit cell angles | 90.00, 97.61, 90.00 |
Refinement procedure
Resolution | 29.865 - 3.241 |
R-factor | 0.1855 |
Rwork | 0.183 |
R-free | 0.22710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nvq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.180 |
Data scaling software | SCALA |
Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.865 | 29.865 | 3.420 |
High resolution limit [Å] | 3.241 | 10.250 | 3.240 |
Rmerge | 0.052 | 0.811 | |
Total number of observations | 12248 | 55439 | |
Number of reflections | 103014 | ||
<I/σ(I)> | 6.6 | 12.5 | 1 |
Completeness [%] | 99.4 | 95.9 | 96.6 |
Redundancy | 3.9 | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 293 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.0, 50mM dioxane, 9-11% PEG 6.000, 15mM DTT, vapor diffusion, temperature 293K |