3S11
Crystal structure of H5N1 influenza virus hemagglutinin, strain 437-10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-17 |
| Detector | MAR CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.365, 241.054, 70.061 |
| Unit cell angles | 90.00, 116.69, 90.00 |
Refinement procedure
| Resolution | 49.070 - 2.500 |
| R-factor | 0.22309 |
| Rwork | 0.221 |
| R-free | 0.26475 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.380 | 2.500 |
| Rmerge | 0.104 | 0.057 | 0.431 |
| Number of reflections | 68017 | ||
| <I/σ(I)> | 10.9 | ||
| Completeness [%] | 96.0 | 99.9 | 72.8 |
| Redundancy | 4.8 | 5.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 23% PEG 3350, 0.1M Tris-Cl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
