3S0Y
The crystal structure of the periplasmic domain of MotB (residues 64-256).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.004 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.210, 75.210, 124.707 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.720 - 1.800 |
| R-factor | 0.1801 |
| Rwork | 0.178 |
| R-free | 0.22140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.325 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.062 |
| Number of reflections | 30716 |
| <I/σ(I)> | 16.8 |
| Completeness [%] | 91.0 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2.1 M ammonium sulphate, 100 mM MES monohydrate pH 6.5 and 2.5% v/v 1,4-dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
