3S06
The crystal structure of the periplasmic domain of Helicobacter pylori MotB (residues 97-256, P3121).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 70.714, 70.714, 143.421 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.1718 |
| Rwork | 0.170 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.325 |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 61.240 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.066 |
| Number of reflections | 38287 |
| Completeness [%] | 98.1 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 100 mM Bicine pH 9.0, 2 M Ammonium Sulphate , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
