3RZH
Duplex Interrogation by a Direct DNA Repair Protein in the Search of Damage
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 200 |
Collection date | 2010-06-16 |
Wavelength(s) | 1.54 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 78.116, 78.116, 229.949 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.250 |
R-factor | 0.2252 |
Rwork | 0.224 |
R-free | 0.25619 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3btx |
RMSD bond length | 0.011 |
RMSD bond angle | 1.541 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 20686 | |
Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 20% PEG 4000, 0.1M sodium chloride, 0.05M magnesium chloride, 0.1M cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |