3RZG
Duplex Interrogation by a Direct DNA Repair Protein in the Search of Damage
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 200 |
| Collection date | 2007-07-03 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.149, 60.980, 65.209 |
| Unit cell angles | 90.00, 89.23, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.620 |
| R-factor | 0.20242 |
| Rwork | 0.201 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3btx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.191 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 48714 | |
| Completeness [%] | 94.3 | 94.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 12% PEG 8000, 0.1M sodium chloride, 0.05M magnesium chloride, 0.1M cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
