3RZ4

Hen egg-white lysozyme in HEPES buffer at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-03-09
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	77.200, 77.200, 36.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.000 - 1.800
Rwork	0.208
R-free	0.22270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fe0
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	17.000		1.850
High resolution limit [Å]	1.800	5.150	1.800
Number of reflections	10731
<I/σ(I)>	31.6	81.86	4.45
Completeness [%]	97.0	98.9	86.7
Redundancy	6.5	6.1	3.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5		0.8M potassium sodium tartrate tetrahydrate, 0.1M HEPES sodium, pH 7.5, VAPOR DIFFUSION, HANGING DROP