3RYL

Dimerization domain of Vibrio parahemolyticus VopL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-29
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 21
Unit cell lengths	56.930, 90.490, 101.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.650 - 3.100
R-factor	0.2459
Rwork	0.240
R-free	0.29360
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.512
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.650	3.200
High resolution limit [Å]	3.100	3.100
Rmerge	0.122	0.426
Number of reflections	10025
<I/σ(I)>	20.56	2.37
Completeness [%]	99.5	99.9
Redundancy	32.75	34.86

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	13-15% PEG3350, 0.2 M sodium fluoride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K