3RW8

Crystal structure of lysozyme in 40% ethanol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-03-31
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	78.216, 78.216, 36.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.979 - 1.855
R-factor	0.1919
Rwork	0.190
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.032
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.880
High resolution limit [Å]	1.855	1.850
Rmerge	0.052	0.152
Number of reflections	10292
<I/σ(I)>	29.6	10
Completeness [%]	96.7	66.7
Redundancy	4.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	277	40mM Sodium acetate, 150mM Sodium chloride, 40% Ethanol, pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K