3RUP
Crystal structure of E.coli biotin carboxylase in complex with two ADP and two Ca ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Detector technology | CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 170.180, 58.843, 85.083 |
Unit cell angles | 90.00, 94.24, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.990 |
R-factor | 0.1736 |
Rwork | 0.171 |
R-free | 0.22690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.312 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 84.820 |
High resolution limit [Å] | 1.990 |
Number of reflections | 56480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | PEG3350, CaCl2, pH 8.5, vapor diffusion, sitting drop, temperature 295K |