3RU5

Silver Metallated Hen Egg White Lysozyme at 1.35 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER IMUS MICROFOCUS
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-28
Detector	APEX II CCD
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	77.798, 77.798, 37.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.550 - 1.350
R-factor	0.19348
Rwork	0.192
R-free	0.21451
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kam
RMSD bond length	0.008
RMSD bond angle	1.175
Data reduction software	SAINT
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.550
High resolution limit [Å]	1.350
Number of reflections	25935
<I/σ(I)>	32.84
Completeness [%]	99.7
Redundancy	18.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	5 mM silver(I) 3-hydroxybutyrate, 3% (w/v) sodium nitrate, 25% (v/v) ethylene glycol, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K