3RSY
Cellobiose phosphorylase from Cellulomonas uda in complex with sulfate and glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-11-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0015 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.100, 103.780, 99.150 |
| Unit cell angles | 90.00, 96.57, 90.00 |
Refinement procedure
| Resolution | 19.976 - 1.810 |
| R-factor | 0.2038 |
| Rwork | 0.202 |
| R-free | 0.23830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.537 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.976 | 1.860 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Number of reflections | 148787 | |
| Completeness [%] | 94.6 | 87.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.8 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
