3RSN

Crystal Structure of the N-terminal region of Human Ash2L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-07
Detector	MAR 300 CCD
Wavelength(s)	0.97856
Spacegroup name	P 31 2 1
Unit cell lengths	49.984, 49.984, 165.519
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.057 - 2.100
R-factor	0.2171
Rwork	0.213
R-free	0.25390
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.117
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.074
Number of reflections	14752
<I/σ(I)>	73
Completeness [%]	99.4	97.9
Redundancy	19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	100 mM Tris-HCl pH8.5, 20% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K