3RRK
Crystal structure of the cytoplasmic N-terminal domain of subunit I, homolog of subunit a, of V-ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97926 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 76.320, 102.181, 139.062 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.620 - 2.640 |
| R-factor | 0.193 |
| Rwork | 0.190 |
| R-free | 0.25500 |
| Structure solution method | SAD |
| Starting model (for MR) | SAD phasing |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.096 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.620 | 2.690 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.049 | 0.469 |
| Number of reflections | 16308 | |
| <I/σ(I)> | 29.4 | 3.1 |
| Completeness [%] | 98.1 | |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.2 M lithium sulfate, 0.8 M sodium-potassium trtarate, 0.1 M CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
