3RR7
Binary Structure of the large fragment of Taq DNA polymerase bound to an abasic site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.460, 109.460, 90.427 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.398 - 1.950 |
| R-factor | 0.1951 |
| Rwork | 0.193 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lwl |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.739 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (dev_704) |
| Refinement software | PHENIX ((phenix.refine: dev_704)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.109 | 0.882 |
| <I/σ(I)> | 10.29 | 1.27 |
| Completeness [%] | 98.9 | 93.6 |
| Redundancy | 5.11 | 4.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M TRIS HCl pH 7.5, 0.2 M Magnesium Formate, 12% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
