3RQW
Crystal structure of acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Detector technology | PIXEL |
| Collection date | 2011-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.763, 266.068, 111.162 |
| Unit cell angles | 90.00, 107.82, 90.00 |
Refinement procedure
| Resolution | 24.977 - 2.913 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vl0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.144 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.800 |
| High resolution limit [Å] | 2.910 |
| Rmerge | 0.044 |
| Number of reflections | 124625 |
| <I/σ(I)> | 22.1 |
| Completeness [%] | 98.0 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 277 | 12% PEG4000, 0.1 M MES, 0.2 M ammonium sulfate, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
