3RQR

Crystal structure of the RYR domain of the rabbit ryanodine receptor

Experimental procedure

Experimental method	SAD
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-04-10
Detector	RIGAKU RAXIS IV++
Spacegroup name	P 43 21 2
Unit cell lengths	68.770, 68.770, 91.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.930 - 2.160
R-factor	0.238
Rwork	0.231
R-free	0.25800
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXS
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.160	2.160
Rmerge	0.103	0.846
Number of reflections	12353
<I/σ(I)>	19.7	2
Completeness [%]	100.0	100
Redundancy	9.2	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	291	1.2M Na Citrate, 100mM Tris pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1		8.5	291	1.2M Na Citrate, 100mM Tris pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K