3RQC
Crystal structure of the catalytic core of the 2-oxoacid dehydrogenase multienzyme complex from Thermoplasma acidophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9702 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 204.840, 204.840, 441.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.950 - 4.010 |
| R-factor | 0.25672 |
| Rwork | 0.253 |
| R-free | 0.32773 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eaf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.806 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.950 | 4.140 |
| High resolution limit [Å] | 4.010 | 4.000 |
| Number of reflections | 28168 | |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 15% MPD, 0.2M NaCl, 0.1M Na Acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
