3RQB
Crystal Structure of Conserved Protein of Unknown Function with Hot dog Fold from Alicyclobacillus acidocaldarius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 109.645, 109.645, 192.614 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.478 - 2.800 |
| R-factor | 0.179 |
| Rwork | 0.175 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.243 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.480 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 17458 | |
| <I/σ(I)> | 6.4 | 2.4 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 8.1 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.2 M Calcium Acetate, 0.1 M Sodium Acetate:Acetic Acid pH 4.5 30% (v/v) PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
