3RPK
Structure of the Full-Length Major Pilin RrgB from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 84.370, 107.930, 142.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.470 - 2.800 |
| R-factor | 0.2206 |
| Rwork | 0.217 |
| R-free | 0.28050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x9w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.320 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 30964 | |
| <I/σ(I)> | 5.6 | 2.1 |
| Completeness [%] | 95.5 | 97.5 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1.9M ammonium sulfate, 0.1M Tris-HCl, 100mM ammonium iodide, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
