3ROF
Crystal Structure of the S. aureus Protein Tyrosine Phosphatase PtpA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2007-10-28 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.810, 35.100, 65.510 |
| Unit cell angles | 90.00, 91.05, 90.00 |
Refinement procedure
| Resolution | 34.804 - 1.030 |
| R-factor | 0.1505 |
| Rwork | 0.149 |
| R-free | 0.16890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.415 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.3_473) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.804 |
| High resolution limit [Å] | 1.030 |
| Number of reflections | 73124 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 0.9 M NaH2PO4/1.1 M K2HPO4 acetate buffer, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 100K |
