3RN4
Crystal structure of iron-substituted Sod2 from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-10-10 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 2 3 |
Unit cell lengths | 92.690, 92.690, 92.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.950 - 1.790 |
R-factor | 0.19829 |
Rwork | 0.196 |
R-free | 0.23791 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bfr |
RMSD bond length | 0.011 |
RMSD bond angle | 1.227 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.950 | 1.890 |
High resolution limit [Å] | 1.790 | 1.790 |
Number of reflections | 25311 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 45% MPD, 0.1M Bicine pH 9.5, 0.2M NH4H2PO4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |