3RMY
Crystal structure of HCR/D W1238A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-10-15 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.519, 115.623, 107.179 |
| Unit cell angles | 90.00, 91.90, 90.00 |
Refinement procedure
| Resolution | 36.600 - 2.300 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n7j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MERLOT |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.600 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 102248 | |
| <I/σ(I)> | 32.1 | 2.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 292 | 0.1 M Pipes pH 6.5, 1 M Malonate, 2.5% MPD, VAPOR DIFFUSION, temperature 292K |
