3RKY

Structural characterisation of staphylococcus aureus biotin protein ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.99510
Spacegroup name	P 42 21 2
Unit cell lengths	94.266, 94.266, 130.885
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.797 - 3.232
R-factor	0.1942
Rwork	0.192
R-free	0.23920
Starting model (for MR)	3rir
RMSD bond length	0.015
RMSD bond angle	1.868
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.200
Number of reflections	9929
Completeness [%]	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	PEG 3350, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K