3RKU
Substrate Fingerprint and the Structure of NADP+ Dependent Serine Dehydrogenase from Saccharomyces cerevisiae complexed with NADP+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-09-08 |
| Detector | RIGAKU SATURN 944+ |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.390, 87.250, 83.810 |
| Unit cell angles | 90.00, 112.98, 90.00 |
Refinement procedure
| Resolution | 40.949 - 2.600 |
| R-factor | 0.1913 |
| Rwork | 0.189 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nwq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.280 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 77.161 | 45.870 | 2.440 |
| High resolution limit [Å] | 2.360 | 5.080 | 2.360 |
| Rmerge | 0.135 | 0.095 | 0.489 |
| Total number of observations | 12307 | 11864 | |
| Number of reflections | 44993 | ||
| <I/σ(I)> | 6.3 | 9.8 | 2 |
| Completeness [%] | 99.9 | 99.7 | 99.8 |
| Redundancy | 4.06 | 4.04 | 3.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10% v/v MPD, 24% w/v PEG4000, 0.1 M ammonium thiocyanate, Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
