3RKO
Crystal structure of the membrane domain of respiratory complex I from E. coli at 3.0 angstrom resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9789, 0.9790, 0.85, 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 94.980, 116.570, 191.370 |
| Unit cell angles | 98.51, 104.08, 108.51 |
Refinement procedure
| Resolution | 19.991 - 3.000 |
| R-factor | 0.2329 |
| Rwork | 0.232 |
| R-free | 0.28160 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.070 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.979 | 29.979 | 3.160 |
| High resolution limit [Å] | 3.000 | 9.490 | 3.000 |
| Total number of observations | 11318 | 52731 | |
| Number of reflections | 139150 | ||
| <I/σ(I)> | 4.2 | 15.3 | 0.8 |
| Completeness [%] | 95.5 | 90.4 | 96 |
| Redundancy | 2.6 | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 296 | 0.1M sodium acetate, 0.3M sodium tartrate, 9% PEG4000, 0.15% sodium cholate, 0.1M NDSB-256, pH 4.8, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
