3RK8
Crystal structure of the chloride inhibited dihydrodipicolinate synthase from Acinetobacter baumannii complexed with pyruvate at 1.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2011-02-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.251, 122.266, 52.564 |
| Unit cell angles | 90.00, 116.31, 90.00 |
Refinement procedure
| Resolution | 47.120 - 1.800 |
| R-factor | 0.15016 |
| Rwork | 0.148 |
| R-free | 0.18824 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pud |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 53625 | |
| <I/σ(I)> | 23.1 | 3.9 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.2M MgCl2, PEG 3350, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
