3RJP
Crystal structure of the DNA binding domain of CovR from Streptococcus pyogenes
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979547, 1.019859, 0.979663 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.467, 36.686, 38.937 |
| Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
| Resolution | 19.590 - 1.500 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.21900 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.660 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 13184 | |
| Completeness [%] | 94.8 | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 14% PEG3350, 50mM Zn(OAc)2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
