3RJ2
Structural and functional characterization of a novel histone H3 binding protein ORF158L from the Singapore grouper iridovirus (SGIV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2007-10-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.066, 41.002, 52.386 |
| Unit cell angles | 90.00, 95.33, 90.00 |
Refinement procedure
| Resolution | 29.900 - 2.200 |
| R-factor | 0.20101 |
| Rwork | 0.197 |
| R-free | 0.27503 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.386 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.000 | |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 6586 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 3% glycerol, 18% PEG3350, 0.1M Tris, 0.3M NaAc, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
