3RIU
Crystal structure of Drosophila hexameric C3PO formed by truncated Translin and Trax
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 196.030, 196.030, 155.189 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.881 - 3.400 |
| R-factor | 0.2739 |
| Rwork | 0.271 |
| R-free | 0.33810 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.864 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.214 | 0.865 |
| Number of reflections | 24690 | |
| <I/σ(I)> | 11.4 | 2.7 |
| Completeness [%] | 99.8 | |
| Redundancy | 14.4 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.35 M-0.7 M (NH4)2HPO4, 0.1 M Na-citrate pH 5.4-5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
