3RIM
Crystal structure of mycobacterium tuberculosis Transketolase (Rv1449c)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 74.530, 80.120, 130.100 |
| Unit cell angles | 82.20, 81.16, 66.37 |
Refinement procedure
| Resolution | 19.810 - 2.490 |
| R-factor | 0.22544 |
| Rwork | 0.223 |
| R-free | 0.27210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | model of Rv1449c with E. coli transketolase (pdb entry 2r8o) as template |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.634 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.120 | 2.640 |
| High resolution limit [Å] | 2.494 | 2.490 |
| Rmerge | 0.590 | |
| Number of reflections | 91429 | |
| <I/σ(I)> | 2.77 | |
| Completeness [%] | 96.6 | 91.16 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293.15 | 17% PEG 10000, 0.1M ammonium acetate in 0.1M bis-tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
