3RIJ
Epitope backbone grafting by computational design for improved presentation of linear epitopes on scaffold proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-01 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 43 |
| Unit cell lengths | 70.000, 70.000, 131.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.000 - 2.300 |
| Rwork | 0.252 |
| R-free | 0.29380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cx5 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 27812 | |
| <I/σ(I)> | 19 | 5.4 |
| Completeness [%] | 99.0 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2.0 M ammonium sulfate, 4% PEG400, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
