3RHT
Crystal structure of type 1 glutamine amidotransferase (GATase1)-like protein from Planctomyces limnophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.287, 126.827, 101.964 |
| Unit cell angles | 90.00, 96.60, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.830 |
| R-factor | 0.15803 |
| Rwork | 0.158 |
| R-free | 0.19172 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.440 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.093 | 0.607 |
| Number of reflections | 93329 | |
| <I/σ(I)> | 19.5 | 2.4 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.16 M calcium acetate, 0.08 M sodium cacodylate, 14.4% PEG8000, 20% glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
