3RHN

HISTIDINE TRIAD NUCLEOTIDE-BINDING PROTEIN (HINT) FROM RABBIT COMPLEXED WITH GMP

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1994-11
Detector	RIGAKU
Spacegroup name	P 31 2 1
Unit cell lengths	50.850, 50.850, 81.830
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.194
Rwork	0.194
R-free	0.25400
Structure solution method	REFINEMENT FROM MIR STRUCTURE OF HINT-ADENOSINE
RMSD bond length	0.011
RMSD bond angle	25.300 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PROTSYS
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.740	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.030 *
Total number of observations	49822 *
Number of reflections	7463 *
<I/σ(I)>	13	5
Completeness [%]	99.0	100
Redundancy	6.7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4 *		30% POLYETHYLENE GLYCOL 8000, 0.1 M SODIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG8000	30 (%)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	sodium cacodylate	0.1 (M)
5	1	drop	GMP	0.2 (mM)
6	1	drop		150 (mM)
7	1	drop	Tris-Cl	20 (mM)	pH7.4