3RHN
HISTIDINE TRIAD NUCLEOTIDE-BINDING PROTEIN (HINT) FROM RABBIT COMPLEXED WITH GMP
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-11 |
| Detector | RIGAKU |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.850, 50.850, 81.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.25400 |
| Structure solution method | REFINEMENT FROM MIR STRUCTURE OF HINT-ADENOSINE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 25.300 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PROTSYS |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.740 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.030 * | |
| Total number of observations | 49822 * | |
| Number of reflections | 7463 * | |
| <I/σ(I)> | 13 | 5 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 6.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.4 * | 30% POLYETHYLENE GLYCOL 8000, 0.1 M SODIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 30 (%) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 5 | 1 | drop | GMP | 0.2 (mM) | |
| 6 | 1 | drop | 150 (mM) | ||
| 7 | 1 | drop | Tris-Cl | 20 (mM) | pH7.4 |
