3RHM
Crystal structure of the E673Q mutant oF C-Terminal domain of 10'FORMYLTETRAHYDROFOLATE DEHYDROGENASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-21 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 261.606, 193.716, 97.315 |
| Unit cell angles | 90.00, 109.05, 90.00 |
Refinement procedure
| Resolution | 49.810 - 2.380 |
| R-factor | 0.17279 |
| Rwork | 0.171 |
| R-free | 0.20225 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o2p |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.161 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.470 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.143 | 0.475 |
| Number of reflections | 176623 | |
| <I/σ(I)> | 6.7 | 1.9 |
| Completeness [%] | 96.9 | 81.2 |
| Redundancy | 4.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES pH 7.0, 1.6M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
