3RHB
Crystal structure of the apo form of glutaredoxin C5 from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 63.527, 39.020, 37.603 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.250 - 1.200 |
R-factor | 0.15029 |
Rwork | 0.150 |
R-free | 0.17585 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fz9 |
RMSD bond length | 0.025 |
RMSD bond angle | 2.209 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.250 |
High resolution limit [Å] | 1.200 |
Rmerge | 0.052 |
Number of reflections | 26715 |
<I/σ(I)> | 21.9 |
Completeness [%] | 94.0 |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 277 | 1.6 M ammonium sulfate, 500 mM lithium chloride, Microbatch, temperature 277K |