3RH8

Crystal Structure of the Light-state Dimer of Fungal Blue-Light Photoreceptor Vivid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	37.647, 77.473, 54.696
Unit cell angles	90.00, 99.77, 90.00

Refinement procedure

Resolution	30.000 - 2.750
Rwork	0.226
R-free	0.28740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 2PD7
RMSD bond length	0.013
RMSD bond angle	2.098
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.750	5.920	2.750
Rmerge	0.157	0.080	0.500
Number of reflections	8222
<I/σ(I)>	4.9
Completeness [%]	98.6	99.1	97.9
Redundancy	3.9	4	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	290	11-15% PEG 6000, 0.1M HEPES (pH = 7.5 - 8.5), 5% 2-Methyl-2,4-pentanediol, VAPOR DIFFUSION, temperature 290K