3RGQ

Crystal Structure of PTPMT1 in complex with PI(5)P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	90.019, 67.914, 32.088
Unit cell angles	90.00, 96.56, 90.00

Refinement procedure

Resolution	44.715 - 2.050
R-factor	0.1748
Rwork	0.173
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.772
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.6.3_473)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.090
High resolution limit [Å]	2.050	5.560	2.050
Rmerge	0.062	0.031	0.240
Number of reflections	11902
<I/σ(I)>	10.2
Completeness [%]	97.8	99.5	92.6
Redundancy	3.7	3.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	PEG 3350, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K