3RG6
Crystal structure of a chaperone-bound assembly intermediate of form I Rubisco
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97908 |
| Spacegroup name | I 4 |
| Unit cell lengths | 244.750, 244.750, 99.669 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.200 |
| R-factor | 0.2175 |
| Rwork | 0.216 |
| R-free | 0.24540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rbl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.073 |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.999 | 47.999 | 3.370 |
| High resolution limit [Å] | 3.200 | 10.120 | 3.200 |
| Rmerge | 0.109 | 0.037 | 0.311 |
| Total number of observations | 5667 | 7961 | |
| Number of reflections | 41265 | ||
| <I/σ(I)> | 8.9 | 14.9 | 2.4 |
| Completeness [%] | 84.8 | 98.7 | 48.7 |
| Redundancy | 3.2 | 3.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.7 | 277 | 15-17 % 2-methyl-2,4-pentanediol, 0.1 M KCl, 0.1 M Tris-HCl, pH 8.7, vapor diffusion, temperature 277K |
