3RG6

Crystal structure of a chaperone-bound assembly intermediate of form I Rubisco

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97908
Spacegroup name	I 4
Unit cell lengths	244.750, 244.750, 99.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.200
R-factor	0.2175
Rwork	0.216
R-free	0.24540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rbl
RMSD bond length	0.007
RMSD bond angle	1.073
Data scaling software	SCALA (3.2.25)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.999	47.999	3.370
High resolution limit [Å]	3.200	10.120	3.200
Rmerge	0.109	0.037	0.311
Total number of observations		5667	7961
Number of reflections	41265
<I/σ(I)>	8.9	14.9	2.4
Completeness [%]	84.8	98.7	48.7
Redundancy	3.2	3.6	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.7	277	15-17 % 2-methyl-2,4-pentanediol, 0.1 M KCl, 0.1 M Tris-HCl, pH 8.7, vapor diffusion, temperature 277K