3RG1
Crystal structure of the RP105/MD-1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 101.509, 141.577, 141.953 |
| Unit cell angles | 94.00, 91.66, 91.37 |
Refinement procedure
| Resolution | 20.000 - 2.910 |
| R-factor | 0.24025 |
| Rwork | 0.238 |
| R-free | 0.27551 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.428 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmerge | 0.063 | 0.457 |
| Number of reflections | 160125 | |
| <I/σ(I)> | 18.2 | 1.8 |
| Completeness [%] | 93.5 | 73 |
| Redundancy | 2.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 296 | 19% PEG8000, 100 mM Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
