3RG0
Structural and functional relationships between the lectin and arm domains of calreticulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-20 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.062, 71.701, 108.386 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.230 - 2.570 |
| R-factor | 0.23663 |
| Rwork | 0.234 |
| R-free | 0.28558 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o0v |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.061 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 10321 | |
| Completeness [%] | 96.9 | 77.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 295 | 25% PEG 1500, 0.1 M MIB buffer, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
