3RFJ
Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.567, 60.836, 87.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.040 - 1.780 |
| R-factor | 0.22366 |
| Rwork | 0.222 |
| R-free | 0.26038 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.279 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.040 | 1.860 |
| High resolution limit [Å] | 1.780 | 1.800 |
| Number of reflections | 27691 | |
| Completeness [%] | 91.2 | 88.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 25.5% polyethylene glycol 4000, 15%(w/v) glycerol, 0.17M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, temperature 293K |
