3RER

Crystal structure of E. coli Hfq in complex with AU6A RNA and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	37.970, 52.380, 55.520
Unit cell angles	106.17, 101.42, 98.95

Refinement procedure

Resolution	32.890 - 1.700
R-factor	0.167
Rwork	0.165
R-free	0.20510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hk9
RMSD bond length	0.010
RMSD bond angle	1.468
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.570	27.529	1.790
High resolution limit [Å]	1.699	5.370	1.700
Rmerge	0.046	0.034	0.138
Rmeas		0.040	0.160
Rpim		0.020	0.080
Total number of observations		4824	23520
Number of reflections	41454
<I/σ(I)>	18.1	31.6	7.4
Completeness [%]	95.8	91.5	94.5
Redundancy	4	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	283	100mM Cacodylate, 100mM NaCl, 12% PEG 8000, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 283K