3RDV

Structure of the SLAIN2c-CLIPCG1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	38.210, 45.740, 49.360
Unit cell angles	100.06, 105.76, 108.39

Refinement procedure

Resolution	45.536 - 1.750
R-factor	0.1836
Rwork	0.180
R-free	0.22880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e3i
RMSD bond length	0.009
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BALBES
Refinement software	PHENIX (dev_279)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.540		1.800
High resolution limit [Å]	1.750	10.000	1.750
Rmerge	0.069	0.036	0.349
Rmeas		0.040	0.382
Number of reflections	28615	155	2254
<I/σ(I)>	17.56	35.95	4.91
Completeness [%]	96.8	98.7	95.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	298	36% PEG 6000, 100mM citric acid, pH 4.5, vapor diffusion, temperature 298K