3RDS

Crystal structure of the refolded R7-2 streptavidin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	I 41 2 2
Unit cell lengths	57.469, 57.469, 173.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.130 - 1.500
R-factor	0.1908
Rwork	0.190
R-free	0.21420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rdm
RMSD bond length	0.012
RMSD bond angle	1.543
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.550
High resolution limit [Å]	1.500	3.230	1.500
Rmerge	0.054	0.033	0.318
Number of reflections	22001
<I/σ(I)>	14.8
Completeness [%]	92.1	87.7	88.8
Redundancy	7.1	7.1	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	25% PEG 1500, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K