3RCP

Crystal structure of the FAPP1 pleckstrin homology domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-05
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	31.025, 31.025, 216.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.826 - 1.900
R-factor	0.2328
Rwork	0.230
R-free	0.25530
Structure solution method	SAD
RMSD bond length	0.017
RMSD bond angle	1.573
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	HKL2Map
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.712	1.930
High resolution limit [Å]	1.900	1.900
Rmerge		0.517
Number of reflections	15364
<I/σ(I)>		6.7
Completeness [%]	96.4	98.1
Redundancy		9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	291	precipitant solution containing 0.1 M NaAc, pH 4.6, 50mM (NH4)2SO4 and 15% PEG1000., VAPOR DIFFUSION, HANGING DROP, temperature 291K