3RC3
Human Mitochondrial Helicase Suv3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 89.390, 89.390, 88.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.840 - 2.080 |
| R-factor | 0.161 |
| Rwork | 0.160 |
| R-free | 0.20300 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.021 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.057 | 0.326 |
| Number of reflections | 47146 | |
| <I/σ(I)> | 15 | 3.49 |
| Completeness [%] | 99.6 | 96.5 |
| Redundancy | 3.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 277 | 6% PEG 3350, 0.1 M Na citrate, 10 % glycerol, pH 6.5, vapor diffusion, hanging drop, temperature 277K |
