3RBC
Bullfrog M ferritin with iron(III) bound to the ferroxidase site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2915 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 210.719, 210.719, 324.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.130 - 2.700 |
| R-factor | 0.176 |
| Rwork | 0.173 |
| R-free | 0.23465 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mfr |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.856 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.880 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.393 | |
| Number of reflections | 210135 | |
| <I/σ(I)> | 5 | 3.3 |
| Completeness [%] | 92.4 | 89.1 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 3M sodium formate, 8% v/v ethylene glycol,20 mM TRIS.HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
