3RBA
Phosphopantetheine adenylyltransferase from Mycobacterium tuberculosis complexed with DPCoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.8 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 99.224, 99.224, 115.852 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 11.406 - 1.590 |
| R-factor | 0.1592 |
| Rwork | 0.157 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | code 1TFU |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.004 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.049 |
| Number of reflections | 35955 |
| Completeness [%] | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | counter diffusion |
