3RB9
Crystal structure of the M. tuberculosis beta clamp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.903, 233.247, 124.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.810 - 3.000 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.25030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.424 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.100 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 21391 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 9% Isopropanol, 12% PEG 4000, 0.1M Na HEPES , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
